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Fig. 6. Expression of Rock in mouse at E11-E13. At E11 (A,A'), Rock1 mRNA could be detected by in situ hybridization in the ventricular (short arrow) and subventricular zone (large arrow) of the rhombic lip. Note the presence of Rock1-positive cells in the early migratory pathway (arrowheads in A'). At E13 (B), Rock1 expression could be detected in ION (asterisk) and in migrating LRN (arrowhead). The floor plate (fp) showed strong Rock1 expression (A,B). (C,D) Western blot illustrating the presence of Rock1 and Rock2 proteins in rhombic lip at E11 and E12 (C), as well as in the LRN and ION domains at E13 (D). Scale bars: A, 180 µm; A', 90 µm; B, 100 µm.





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