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Fig. 2. Fgf signalling dorsalises the embryo by inhibiting Bmp gene expression.
(A-D) Inhibition of Bmp signalling or Fgf overexpression cause dorsalisation
phenotypes. Early segmentation stage morphology of wild-type (A),
nog1-injected (B), fgf3-injected (C) and
fgf24-injected (D) embryos. (E-N) Blastula stage Bmp gene expression
is unaffected by inactivation of Bmp proteins, but lost following Fgf
overexpression. All embryos are at 30% epiboly. Expression of bmp2b
in wild-type (E), nog1-injected (F) and fgf8-injected (G,H)
embryos (H is a lateral view of G, dorsal toward the right). (I,J)
bmp7 expression in wild-type (I) and fgf8-injected embryos
(J). (K,L) bmp4 expression in wild-type (K) and
fgf8-injected embryos (L). (M,N) Loss of bmp2b expression
following injection of fgf3 (M) or fgf24 (N). (O,P)
fgf8 overexpression dorsalises chd-depleted embryos.
Characteristic dorsalised morphology (O) and loss of bmp2b expression
at blastula stage (P) following co-injection of mo-chd and
fgf8 RNA. (Q,S) Ectopic expression of spry4 expression after
localised misexpression of RNA encoding Erm1 (Q) or Pea3 (S). (R,T) Local
inhibition of bmp2b expression (asterisk) after localised
misexpression of RNA encoding Erm1 (R) or Pea3 (T). (A-D,O) Dorsal views,
anterior towards the left; (E-G,I-N,P-T) Animal pole views, dorsal towards the
right.
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