spacer gif spacer gif spacer gif spacer gif ARCHIVE ANNOUNCEMENT! spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 4. Spry2 is a feedback inhibitor of Fgf signalling. (A) Wild-type expression of spry2. (B) Inhibition of Fgf signalling by injection of RNA encoding dn-fgfr abolishes spry2 expression. (C) Ubiquitous or (D) localised (arrowhead) overexpression of fgf8 RNA induces ectopic spry2 expression. (F) spry2 expression is reduced in fgf8/ace mutant embryos compared with wild-type siblings (E). Compared with wild-type (G) embryos injected with fgf8 RNA (H) display dorsalised phenotypes. (I) Co-injection of fgf8 with spry2 RNA rescues embryos. (J,O,L) Injection of (J) mo-spry2 or (O) dn-spry2, or (L) co-injection of both induce dorsalised phenotypes. Compare with injection of fgf8 RNA (H,P). (K) Dorsalisation is abolished by co-injection of mut-spry2. (A-D) Late blastula stage, (E-P) early segmentation stage. (A-C) Animal pole views; (D-L) lateral views; (M-P) dorsal views. (E,F,M-P) Anterior is towards the left.





Right arrow Return to article