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Fig. 7. Inhibition of Bmp signalling restores the ectodermal DV patterning in
Fgf-depleted embryos. Analysis of the DV patterning of the ectoderm with
foxi1 (A-D), cyp26a (E-H) and vhnf1 (I-L). Black
arrows indicate the space between the blastoderm margin and the ectodermal
gene expressions. (B) Microinjection of dn-ras causes an expansion of
foxi1 expression, (F) a reduction of the neural expression domain of
cyp26a (white arrowhead) and (J) a complete loss of vhnf1.
(C) Co-injection of nog1 with dn-ras abolishes epidermal cell fates,
(G) expands the anterior neurectoderm but (K) does not rescue vhnf1
expression. (D) nog1 injection abolishes foxi1 expression,
while the neural expression domains of cyp26 (H) and vhnf1
(L) expand ventrally. (A-H) 75% epiboly stage; (I-L) 85% epiboly stage. All
embryos are shown in lateral view, dorsal towards the right.
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