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Fig. 2. Ectopic Ptc1 expression is localised to diastema mesenchyme. (A-D)
E11.5 mandibular mesenchyme cultured for 24 hours. (A) Barx1 marks
the molar regions. (B) Double-labelled (digoxigenin-fluorescein) in situ
hybridisation marks Ptc1 (blue staining) diastema regions as distinct
from Barx1-expressing (red staining) molar regions. (C) Adjacent
sections through Ptc1-expressing domain (upper) demonstrate an
absence of Barx1 (lower). (D) Adjacent sections through
Barx1-expressing molar region (upper) demonstrate an absence of
Ptc1 (lower). (E-H) Ectopic expression of Ptc1 and
Gli1 in the diastema is due to Shh signalling. E11.5 mandibular
mesenchyme cultured for 24 hours in the presence of 2H3 control antibody (E,G;
normal Ptc1, Gli1 expression, respectively) or 5E1 Shh-blocking
antibody (F,H; loss of Ptc1, Gli1 expression, respectively). (I,J)
E11.5 mandibular explants cultured from Pro
1(II)-lacZ transgenic mice
demonstrating lacZ expression in Meckel's cartilage. (I) In the
presence of epithelium Meckel's cartilage extends as two symmetrical rods. (J)
In the absence of epithelium Meckel's cartilage extends further distally
(arrowed), but no ectopic cartilages are visible after 24 hours. (K,L)
Ihh expression is absent at E11.5 in the mandibular arch in both the
presence (K) and absence (L) of epithelium after 24 hours. Scale bars: in A,
600 µm for A,B,E-L; in C, 600 µm for C,D.
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