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Fig. 6. Expression and regulation of Gas1 in the murine first arch. (A,B)
Coronal sections through the branchial regions at E11.5 (downregulation of
Gas1 in odontogenic mesenchyme arrowed). (C-L) Timed culture of E11.5
mandibular mesenchyme. (C-F) Gas1 progressively downregulates in the
diastema (arrowed), whereas expression is maintained in the non-odontogenic
mesenchyme underlying the incisor and molar regions. (G-L). Adjacent section
in situ hybridisation demonstrates progressive Gas1 downregulation
(G,I,K) and Ptc1 upregulation (H,J,L) in isolated diastema mesenchyme
between 18 and 24 hours of culture. (M-P) E11.5 mandibular mesenchyme cultured
for 24 hours following co-electroporation of Gas1 and GFP
constructs (M,N), or electroporation of a GFP construct alone (O,P).
Electroporation fluorescence (M,O); Ptc1 expression (N,P). Note
downregulation of Ptc1 in the presence of ectopic Gas1
expression (N, arrowed), as opposed to controls (P). Scale bars: in A, 200
µm for A,B; in C, 600 µm for C-F,M-P; in G, 600 µm for G-L.
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