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Fig. 1. nlk encodes a protein that is highly homologous to other
vertebrate Nlks, and is expressed broadly throughout development. (A) CLUSTAL
alignment of zebrafish, Xenopus and human Nlk proteins. Identical
amino acids are shaded in dark gray; similar residues are in light gray. All
three proteins are extremely highly conserved throughout most of the sequence,
but are highly divergent near the N terminus. Overall, zebrafish Nlk is most
similar to Xenopus Nlk. (B-F) nlk in situ hybridization. (B)
Lateral view of a shield stage embryo, dorsal to the right, showing ubiquitous
nlk expression. (C) Dorsal view of a tailbud stage embryo.
nlk is broadly expressed, but higher levels are seen in the
prospective notochord (arrowhead). (D) Dorsal view of a flat-mounted embryo at
15 somites. nlk is highly expressed throughout the CNS, excluding the
developing eyes. (E) Dorsal view of a flattened embryo at 24 hours shows
intense staining in the eyes, in addition to continued strong staining
elsewhere in the CNS. (F) Ventral view of a 48 hpf embryo. nlk
transcripts are localized to the otic vesicles (arrowhead), and in the
ganglion cell layer of the retina (arrow).
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