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Fig. 3. nlk RNA represses anterior neural fate in a
wnt8-dependent manner. (A-F) 90-95% epiboly, lateral view, dorsal to
the right. Embryos are stained with a mix of opl and tbx6
probes. Injection of 200 pg nlk RNA (B) or 10 pg wnt8 RNA
(C) represses expression of opl (arrowheads) in the prospective
telencephalon, compared with wild type (A). Injection of 1 ng each of
wnt8 ORF1 and ORF2 morpholinos (MOs) causes a posterior shift of
opl expression towards the margin (D, arrowhead). Co-injection of a
control MO with nlk RNA has no effect on opl (E), whereas
injection of wnt8 MOs restores opl expression when
co-injected with nlk RNA (F). (G,H) Twenty-four-hour embryos, lateral
view, showing rescue of eyes in nlk RNA + wnt8 MOs (G)
versus nlk RNA + control MO (H).
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