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Fig. 1. nmo expression in the wing imaginal disc was examined in
nmo-lacZ. (A) In second instar discs, weak nmo expression is
seen at the periphery of the future wing pouch (arrow). (B) In early third
instar discs, low level expression is at the DV boundary (arrow) and
encircling the wing pouch. (C) In late third instar, high levels of
nmo expression are seen in two stripes flanking the DV boundary and
in a ring around the pouch. nmo is also seen in the L3, L4 and L5
vein primordia (arrowheads) and in several spots in the presumptive notum
(arrow). (D) In situ hybridization using an antisense nmo RNA probe.
(E-G) Co-localization with Wg. Discs were double stained with (E)
anti-ß-gal and (G) anti-Wg antibodies and the images were merged to show
overlap (F). Wing imaginal discs are orientated anterior towards the left,
dorsal side upwards.
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