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Fig. 1. Transcriptional targets of Sox17ß. Embryos were injected at the
two-cell stage with increasing doses mRNA of encoding Sox17ß (62 pg, 125
pg, 250 pg, 500 pg), animal cap explants were isolated at blastula (stage 9),
cultured until gastrula (stage 11) and assayed for endodermal gene expression
by RT-PCR. None of the endodermal genes was expressed in uninjected animal cap
tissue (–), but all were expressed in gastrula whole embryos.
Ef1
, is a loading control; –RT, without reverse
transcriptase; +RT, with reverse transcriptase.
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