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Fig. 7. NS-divisions induced by retrovirus-mediated Ngn2 expression. (A) Retroviral
vectors used in this study. (B-D) Section of an E14 cerebral wall infected
with the control-GFP virus 48 hours earlier, doubly stained with anti-GFP and
anti-pH3 showing two GFP+ mitoses at the ventricular surface
(arrows). (E-G) Section of an E14 cerebral wall infected with the ngn2/GFP
virus 48 hours earlier showing two GFP+ mitoses in the NS position
(arrows). (H) Retroviral infection leads to expression of Ngn2 by a
GFP+ mitosis at the NS position of a ngn2-introduced cerebral wall.
(I) NS mitoses are significantly increased (P<0.001 compared with
control) in ngn2 retrovirus-treated samples (n=7 for control-GFP,
n=9 for ngn2/GFP; mean±s.e.m.). Scale bars: 10 µm in
B,C,E,F,H. CMV, cytomegalovirus promoter; IRES, internal ribosomal entry site;
GFP, green fluorescent protein; LTR, long terminal repeat.
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