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Fig. 2. Expression patterns of AP1, WUS, and HEN3 in plants. (A,B) In situ hybridization on sections of stage 3 flowers to detect AP1 RNA. (A) A hua1-1 hua2-1 flower. (B) A hua1-1 hua2-1 hen3-1 flower. AP1 RNA is found throughout the flower in hua1-1 hua2-1 hen3-1. (C,D) In situ hybridization on sections of stage 9 flowers to detect WUS RNA. (C) A stage 9 hua1-1 hua2-1 flower that has ceased to express WUS. (D) A stage 9 hua1-1 hua2-1 hen3-1 flower that still expresses WUS (arrow). (E-H) GUS staining of HEN3-GUS transgenic lines. HEN3-GUS is found in proliferating tissues or organs. (E) HEN3-GUS is present at the root tip and lateral root primordia. (F) HEN3-GUS is present at the shoot tip, in young leaves, and at the base of growing leaves. (G) HEN3-GUS is present in young flowers. (H) HEN3-GUS is found throughout a stage 7 flower. (I,J) In situ hybridization on longitudinal sections of wild-type inflorescences using either the sense (I) or the antisense (J) HEN3 probe. HEN3 RNA is found at a low level throughout the inflorescence meristem and the floral meristems. Scale bars: 50 µm.





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