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Fig. 3. AP1, AG and HEN3 expression in various genotypes. (A) RNA filter hybridization to examine AG and AP1 RNA levels in various genotypes as indicated. The two AG RNAs containing 2nd intron sequences are marked as RNA1 and RNA2. The abundance of AP1 and AG RNAs is indicated by the numbers below the gel images. (B) Western blotting on total proteins from inflorescences from various genotypes using anti-AG antisera. ag-3 serves as a negative control. AG protein abundance is similar in hua1-1 hua2-1 and hua1-1 hua2-1 hen3-1 flowers. The band marked by an asterisk is a crossreacting, non-AG species from the same blot. (C) RNA filter hybridization to determine the abundance of HEN3 RNA from inflorescences of various genotypes. The abundance relative to wild type is indicated by numbers below the gel images.





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