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Fig. 2. Fzr is partially sufficient to induce precocious endocycles, dMyc to
accelerate endocycles. Ectopic expression of fzr (hsFlp; UAS-fzr;
UASGFP act<FRT-CD2-FRT<Gal4) reduces CycA and B levels, stops
mitosis (A,B) and allows the formation of large nuclei (B) indicative of
precocious endocycles prior to stage 6 in oogenesis. The diagram in C presents
the percentage of the wild-type and mutant follicle cells that show positive
staining to mitotic markers during the mitotic stage. fzr (D,F,J) and
myc (E,G,K) overexpression (hsFlp; UAS-fzr or UAS-myc; UASGFP
act<FRT-CD2-FRT<Gal4) generate enlarged nuclei, which is indicative
of abnormal endocycles but do not affect the amplification stage because
normal BrdU-incorporation pattern at stage 10B-12 (red arrows) is observed in
cells overexpressing fzr (F) and myc (G). (H-K) FACS
profiles of DNA content in UAS-fzr (J) and UAS-myc (K)
mutant nuclei compared with these in WT nuclei (H) show the appearance of a
cell population that has 32n DNA, also observed in Rbf mutants (I)
(Bosco et al., 2001). Green,
GFP (A,D,E), Fzr Ab (F); red, CycB (A), DAPI (D,E), BrdU (F,G); blue, DAPI
(A,F,G).
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