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Fig. 2. Recombination of the Gata1 floxed locus. (A) Maps of the floxed and the knockout Gata1 locus after recombination. N, NcoI; B, BamHI, X, XbaI; En2, murine engrailed 2 intronic sequence; SA, splice acceptor; GFP, green fluorescence protein. LoxP sites are indicated. (B) DNA samples of 13.5 dpc foetuses were digested with NcoI and blotted against the En2 probe for quantifying recombination of the Gata1 floxed locus. 1, XY wild-type male head DNA control; 2, XFLX female head DNA control; 3, XKOX female foetal liver DNA.





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