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Fig. 4. (A) CASY analysis: the ratio of definitive versus primitive erythrocytes in foetal blood was compared at 13.5 and 14.0 dpc. The percentage of definitive cells in blood in each genotype at 13.5 dpc and 14.0 dpc is depicted below the graph. Average and s.d. are indicated. At least three foetuses were analysed per group. (B) CASY analysis: example of CASY graphs from wild-type male and compound female at 13.5 dpc. Peaks corresponding to enucleated and nucleated cells are indicated. The peak below 5 µm corresponds to cell debris. (C) Blood cytospins of each genotype at 13.5 dpc showing primitive nucleated erythrocyte (1), definitive enucleated erythrocyte (2), erythroid precursor (arrowhead). Pictures were taken at 100x magnification.





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