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Fig. 5. (A) Foetal liver cytospins of each genotype at 14.0 dpc. Consecutive differentiation stages are indicated in photograph c and f: large proerythroblast (1), basophilic erythroblast (2), polychromatic erythroblast (3) (which exhibit both basophilia and benzidine positivity), orthochromatic erythroblast (4) (which are strongly benzidine positive) and enucleated definitive erythrocyte (5). Pictures were taken at 100x magnification. (B,C) FACS analysis of (B)13.5 and (C) 14.0 dpc foetal liver cells at the day of collection. Percentage of alive erythroid (TER119+/7AAD–), alive non-erythroid (TER119–/7AAD–), dead erythroid (TER119+/7AAD+) and dead non-erythroid (TER119–/7AAD+) cells are depicted. Average and s.d. are indicated. At least three foetuses were analysed per group.





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