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Fig. 7. Gata1 levels decrease during terminal erythroid differentiation. (A) Whole
cell extracts from wild-type foetal liver cells, after day 0, 1 and 2 of
hanging drop culture were analysed by western blotting using antibodies
against Gata1 with nucleophosmin as loading control. Two samples at day 0 are
shown and cultures were done in triplicate. (B) Jo2 treatment of wild-type
cells. Whole cell extracts at day 2 after standard (St) culture and culture
with Jo2 1 (20 µg/ml) or Jo2 2 (40 µg/ml) were analysed by western
blotting using antibodies against Gata1, and nucleophosmin as a loading
control. (C) Jo2 treatment of erythroid cells from Gata1-overexpressing
foetuses. Whole cell extracts at day 0 and 2 after standard culture and
culture with Jo2 1 (20 µg/ml) were analysed by western blotting using
antibodies against Gata1 with nucleophosmin as a loading control. Extracts
from wild-type, XOXX female and XOXY male foetal liver
cells are shown.
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