DEV01261 Supplementary Material

Files in this Data Supplement:

• Movie 1 -

  Movie 1. Dynamics of cell behavior and morphogenesis in SAMs over 66 hours. The three-dimensional time lapse series of an SAM expressing 35S::H2B:mYFP was acquired at 75minute intervals. Forty-six out of 49 such serial time points are animated at 10 frames/second; three time points have been removed from the time-lapse series due to poor alignment. The movie loops after each cycle. Arrows indicate developing primordia at different stages. Watch P1 for a view of the initial co-ordinated cell expansion, followed by a burst of cell division.

• Movie 2 -

  Movie 2. Dynamics of cell division, cell expansion and cell behavior at the boundary region of SAMs. The three-dimensional time lapse series of an SAM expressing 35S::YFP29-1 was acquired approximately every 2 hours and 30 minutes for a total duration of 65 hours. Twenty-four observations are animated at 10 frames/second. The movie loops after each cycle. The red open arrow indicates cells that form the boundary region. The red closed arrow indicates cells in a primordium. The white arrow indicates cells in the PZ, dividing along their axis of expansion. The yellow arrow indicates a cell in the CZ dividing along its short axis. Other cells in the CZ show proportional expansion in all directions.