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Fig. 5. E4BP4 is not regulated by survival signalling pathways in motoneurons.
Semi-quantitative RT-PCR and western-blot analysis of E4BP4 expression in
cultured motoneurons in the presence or absence of trophic support 6 hours
after plating. (A) Radioactive Southern-blot analysis of RT-PCR amplified
transcripts. (B) Western blots showing E4BP4 and 
-tubulin expression.
(C) Relative band intensities of RT-PCR and Western-blot analysis. Each band
was scanned and its intensity analysed by Image J software. Each single E4BP4
band was normalised with respect to its corresponding actin or tubulin control
band and then the values for parallel cultures with or without trophic factors
were expressed as an intensity ratio (see Materials and methods). Mean values
are plotted (mean±s.e.m.; n=30 independent RT-PCR experiments
and n=10 western blots).
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