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Fig. 2. fat mutant clones upregulate targets of distal-to-proximal signalling. In this and subsequent figures, all panels show third instar wing discs, oriented with ventral down and anterior left, and panels marked prime show separate stains of the same disc. Clones of cells mutant for fat⁸ are marked by the absence of MYC (green). Arrows indicate clones with ectopic gene expression. Discs are stained for WG (red), rn-lacZ (blue/white in C, red in E), spd-fg-lacZ (blue/white in D, red in F), and NUB (blue). (A) Early third instar disc. (B) Late third instar disc. (C) Mid-third instar disc. Ectopic WG expression is associated with an expansion of the rn domain. (D) Mid-late third instar disc. The spd-fg enhancer and endogenous WG are both ectopically expressed, although differences in subcellular localization result in apparent differences within a focal plane. (E) Late third instar disc with a fat clone that extends beyond the NUB domain; arrows here point to the edges of the clone where it extends proximally; rn is induced only within NUB-expressing cells. (F) Mid-third instar disc with a fat clone in the NUB domain with spd-fg-lacZ expression (arrow), and a clone just proximal to this without spd-fg-lacZ expression (asterisk).

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