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Fig. 7. Four-jointed can influence gene expression in the proximal wing.
FJ-expressing clones are marked by GFP (green). (A) Late third instar disc,
stained for fj-lacZ (blue/white) and WG (red). Arrow points to a
clone that induces WG expression in flanking cells in the proximal wing. No
induction of fj occurs. (B) Mid-late third instar disc, stained for
VG (blue/white) and WG (red). Arrows point to clones that induce WG. No
induction of VG occurs. (C) Mid-third instar disc, stained for expression of
rn-lacZ (blue/white) and WG (red). Arrow indicates induction of WG
and rn flanking a clone. Their expression is also decreased within
the FJ-expressing cells. (D) Late third instar disc, stained for
rn-lacZ (blue) and WG (red). Arrows point to clones that alter WG
expression. (E) Mid-late third instar disc, stained for NUB (blue) and
spd-fg-lacZ (red). spd-fg-lacZ is induced only up to the
edge of the NUB domain, even though the clone (arrow) extends proximally.
Although spd-fg-lacZ expression is broader and more diffuse than
endogenous WG (Neumann and Cohen,
1996), it does not extend to the edge of NUB expression in the
absence of ectopic FJ. (F) Mid-third instar disc, stained for NUB (blue) and
rn-lacZ (red). rn-lacZ is induced only up to the edge of the
NUB domain, even though the clone (arrow) extends proximally. (G) Early third
instar disc, stained for Fat (magenta). Fat is tightly localized apically;
because the disc is not flat this figure is a composite projection of
different focal planes to allow visualization of Fat over a broad region. Fat
appears to be concentrated along the edge of the clone (arrow).
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