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Fig. 2. Expression patterns of Ser and a Ser wing enhancer in
wing imaginal discs. (A-G) Wing imaginal discs. UAS-nuc-lacZ
expression driven by the Ser wing enhancer construct 5 (Gal4) was
visualized by ß-galactosidase antibody staining (A,C,D). These patterns
are identical to those of the endogenous Ser protein (not shown). Ser
mRNA was detected by in situ hybridization in developing wing discs (E-G).
(A,B,E) Ser mRNA and construct 5 are expressed in dorsal cells in
early third instar. (B) The expression patterns of construct 5
(Gal4)/UAS-GFP (green) and apterous-lacZ (red), are
co-localized (yellow) in dorsal cells of wing and haltere discs. Dorsal is to
the left for the haltere disc. White dashed circles outline the discs. (C,F)
By the mid third instar, construct 5 (Gal4)/UAS-nuc-lacZ and Ser mRNA
are preferentially expressed along the DV border. (D,G) At the end of the
third instar, they are expressed in two stripes flanking the DV boundary, with
higher expression dorsally. They are also expressed in presumptive veins (L3,
L4 and L5). m, margin.
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