|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 5. Temporal analysis of wild-type larval and adult gene expression. (A)
Diagram of germline development and timepoints taken for analysis. Black,
somatic gonad; orange, proliferating germ cells; pink, meiotic germ cells;
red, differentiating spermatocytes; blue, differentiating oocytes. (B)
Temporal expression profiles for all genes with P<0.01 (ANOVA) in
timecourse, organized by similarity in expression using hierarchical
clustering. Each row represents a gene, while each column represents an
average of each set of comparisons. The log2 ratios have been
replaced with color that demonstrates the relative level of expression. Six
clusters, a-f, with different temporal patterns are defined by bars down right
side of clustergram. (C) The mutant expression profiles of the different
genotypes of temporally regulated genes. Genes are present in the same order
as B. The mutant data had no weight in the clustering, but were included to
demonstrate the mutant profile of each genotype. The `fem' column (column 1)
corresponds to the fem-3/fem-1 direct comparison. The + and
- notations refer to the presence or absence of the germline (wild type or
glp-4 mutants, respectively). For both B and C, yellow indicates
higher expression in staged wild-type or mutant sample; blue indicates higher
expression in reference sample or fem-1(lf).
|
