|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 5. Gene expression and patterns of cell death in developing 4C-BAC16 limbs.
(A) Identical pattern of Shh expression in 4C-BAC16 mutant and
wild-type limb buds. White arrow indicates the anterior part of mutant hind
limb. (B-E) Expression patterns of Hoxd13 in the right hind limbs of
2C (B,D) and 4C (C,E) BAC16 mutants. 2C-BAC16 expression patterns resemble
wild type (not shown). Dashed lines (B,C) represent the expected normal
posterioranterior limit of Hoxd13 expression in developing hind limb
buds; limb anterior is towards the bottom of photograph. (F) Medial and (G,H)
anterior views of 2C (G) and 4C (H) E11.5 hind limb buds, showing a reduction
in Dkk1 expression in the anterior two-thirds of the AER (region
between the open arrowhead and black arrowhead) in the 4C-BAC16 limb. The 2C
pattern resembles wild type (not shown) and `I' marks the anterior side of
each limb corresponding to where digit I develops. Arrowheads point to the
ANZ, where Dkk1 expression is completely quenched in the 4C limb bud.
(I) Dorsal view of left and right hind limbs (LHL and RHL) from a 4C-BAC16
mutant stained with Neutral Red. The cell death pattern in this 4C-LHL
resembles that of wild type (not shown). `I' marks the position of developing
digit I condensates and the arrow indicates the absence of Neutral
Red-positive cells in the abnormal (larger digit I) right hind limb, compared
with the presence of such cells in the corresponding region in the normal left
hind limb. Also note the similar, unperturbed, patterns of Neutral Red
staining at the posterior margins of each limb. (J) Comparison of
Wnt5a expression in wild-type and 4C-mutant embryos shows a higher
Wnt5a signal in both fore and hind limbs of 4C-mutants, when compared
with the wild type, particularly in the region generating digit I in the hind
limb buds (arrows). Scale bars: 400 µm in F,I; 600 µm in B,D; 1.0 mm in
J; 1.2 mm in A.
|
