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Fig. 1. Cell movement during proventriculus development. Developing proventriculi
of stage 12 (A,E,I), stage 14 (B,F,J), stage 15 (C,G,K) and stage 17 (D,H,L)
wild-type embryos. (A-D) Anti-Fkh (red)/anti-Dve (green) marking ectodermal
and endodermal cells, respectively. A constriction separates the ectodermal
and endodermal part of the keyhole (arrow in B, see also F). (E-H)
anti-Armadillo staining. Note the concentration of Arm towards the apical side
of the cells (arrow in E,F). (I-L) Anti-Arm(red)/anti-MHC (blue)
immunostaining. The mesoderm-free region that lacks MHC expression is marked
by arrows in I. (M-Q) Schematic representation of the different stages of
proventriculus development and the cell movements resulting in the
invagination of the ectodermal keyhole cells. Ectodermal cells in orange,
endodermal cells in green and mesodermal cells in blue.