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Fig. 3. Ventral narrowing of rhomboid mutants. (A) The ventral cuticle of
thoracic parasegments from a wild-type embryo is shown for reference. The
Keilin's organs (KO) and ventral black dots (vbd) are indicated with
arrowheads, and the denticle belts are labelled to the left of the image. (B)
The distance between KOs and vbds was measured in arbitrary units. This
analysis served as a measure of ventral narrowing and thus of ventrolateral
specification (Mayer and Nusslein-Volhard,
1988). Mutation of rhomboid-3 alone caused no ventral
narrowing, nor did it enhance the narrowing phenotype of rhomboid-1.
Note that rhomboid-1 nulls often lack KOs and as such embryos could
not be scored for this measurement (n is the number of embryos analysed; SE is
standard error). (C) No additional defects in abdominal denticle specification
were evident in rhomboid-3, or rhomboid-3 rhomboid-1 double
mutant embryos compared to wild-type and rhomboid-1 mutants,
respectively.
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