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Fig. 6. Phenotypic analysis of denticle belt fusions during embryogenesis. (A) The
denticle belt fusion phenotype resulted in folds around the surrounding fused
areas (left panel, arrowhead), and in rare events a hole in the cuticle could
be seen at the centre of the fusion (right panel, arrowhead). (B) No defects
could be observed in paired domains of stage 11/12 embryos expressing
DN-EGFR compared to wild-type embryos: paired domains were marked by
co-expression of GFP (green) and Engrailed was used as a segmental marker (in
red). In these and subsequent images anterior is to the right. (C,D) Apoptosis
(detected by TUNEL labelling in green) was elevated predominantly in
paired domains (in red) of ventral epidermal cells of stage 12
embryos (C), and persisted in stage 13 and 14 embryos (D) expressing DN-EGFR
(right panels) compared with wild-type embryos (left panels). (E,F) By stage
15, fusions became evident as curvature of Engrailed stripes laterally
(arrowheads) and missing stripes medially only in paired domains of
embryos expressing DN-EGFR (F shows a merged image of E with paired
domain marked by co-expression of GFP in green, and Engrailed in red). (G) The
cuticle phenotype of embryos expressing DN-EGFR in paired domains
compared to those expressing GFP alone: the only cuticle phenotype of the
DN-EGFR-expressing embryos was strong denticle belt fusions in alternating
parasegments (paired domains).
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