Supplemental Figure 1
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Fig. S1.
Subcellular localization of Ptch1 and Hhip1 with Shh. (A-R) COS-7 cells
expressing Ptch1-YFP, Hhip1-YFP, YFP-GPI or Shh-FLAG, as indicated in each
panel. Red, signal from immunofluorescence with anti-FLAG antibody; green,
fluorescence from YFP; blue, Topro3 staining of nuclei. (A-F) Merged images of
red, green and blue channels. (G-L) Green channel only. (M-R) Red channel only.
(B,H,N) High magnification of boxed area in A. (D,J,P) High magnification of
boxed area in C. (F,L,R) High magnification of boxed area in E.