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Fig. 5. Canonical Wnt signaling impaired in the Ihh-/- embryo. In situ hybridization using digoxigenin-labeled riboprobes against Tcf1 (A,A'), Dkk1 (B,B'), Lef1 (C,C'), Tcf3 (D,D') or Tcf4 (E,E') on sections of E14.5 tibia of wild type (A-E) versus Ihh-/- embryos (A'-E'). Arrows indicate the perichondrium. P, proliferating chondrocytes; PH, prehypertrophic chondrocytes; H, hypertrophic chondrocytes; C, chondrocytes.





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