DEV01829 Supplementary Material

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• Supplemental Figure 1 -

  Fig. S1. Signalling is unaffected in the gut and dorsal ectoderm in cv-c mutants. (A-D) Late stage 14 (A,C) and stage 16 embryos stained for Antennapedia (Antp). Antp is expressed in a region of the visceral muscle that prefigures the site of the anterior midgut constriction (arrowhead in A); expression continues until after the constriction has formed (arrowhead in B). The pattern of Antp expression is not affected in cv-c^C524 embryos (arrowhead in C,D). (E,F) Wild-type (E) and cv-c^M62 (F) embryos undergoing dorsal closure, stained for anti-phosphorylated Mad (pMad) to reveal Dpp pathway activity in lateral epidermal cells. During dorsal closure, leading edge cells secrete Dpp that induces elongation of lateral epithelial cells. pMad (black nuclear staining) can be seen in a row of approximately five cells in wild-type (E) and cv-c (F) embryos (white double arrow), demonstrating that Dpp signalling is unaffected in cv-c mutants.