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Fig. 2. Neural crest migration is dependent on normal non-canonical Wnt signalling.
(A,B) Embryos were injected into the animal blastomeres at the 8-cell stage
with 1 ng of mRNA coding for Dsh-
N (A) or Dsh-DEP+ (B). The embryos were
cultured until stage 24, when the expression of the neural crest marker
Slug was analyzed at postmigratory stages; the injected side (white
arrowhead) was identified by FDX expression (pale green). The uninjected side
shows the normal pattern of cephalic neural crest migration, which is
indicated by the red arrowheads, each one pointing to the mandibular, hyoid
and branchial neural crest, respectively. The injection of
Dsh-N and Dsh-DEP+ led to a dramatic
inhibition of neural crest migration (white arrowhead in A,B; 40%,
n=60, and 45%, n=55, of embryos showed inhibition of neural
crest migration, respectively). (C) One-cell-stage embryos were injected with
mRNA coding for Dsh-DEP+, together with the fluorescent lineage tracer FDX
(green). At the early neurula stage, the prospective cephalic neural crest
were taken from the injected embryos and grafted into a normal uninjected
neurula embryo. The migration of the neural crest was analyzed in vivo by
following the fluorescence label until stage 26, when the cephalic neural
crest has reached its final destination. (D,F) Control embryo showing the
normal pattern of cephalic crest migration; 95% of grafted embryos exhibited
normal migration, n=30. (E,G) Embryo grafted with neural crest taken
from an embryo expressing Dsh-DEP+. No migration of the neural crest is
observed on the operated side. Only 5% of grafted embryos showed normal
migration, n=20.
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