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Fig. 3. bNCSCs generate sensory neurons. (A) Example of a differentiated stem cell clone showing two populations of neurons, one double labelled with peripherin and ßIII-tubulin (Tuj1, yellow) and one only expressing ßIII-tubulin (red). (B) Example of a differentiated stem cell clone produced from the Fkh3lac-z /+ mice stained with X-gal, showing that the bNCSCs give rise to Fkh3+ cells of the sensory lineage. (C) Percentage clones positive for peripherin and Fkh3 (n=58 and 25, respectively). (D) Differentiated bNCSCs stained for CGRP (red) and peripherin (green) showing a mature sensory phenotype. Abbreviations: CGRP, calcitonin gene-related peptide Scale bars: in 100 µm in A,B; 25 µm in D.





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