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Fig. 6. bNCSC-derived sensory neurons are physiologically functional and of
different subclasses. Kinetic profiles of Ca2+ transients of single
cells in bNCSC cultures, as well as in dissociated adult dorsal root ganglion
cells. Each track indicates the change of the ratiometric fluorescence signal
(
F) from a single neuron during its response to stimuli. The duration
of the stimuli is depicted underneath the curve by black horizontal bars. (A)
Three different cell types with a differential response to capsaicin and
hypo-osmolarity showing the presence of both mechanoinsensitive and
mechanosensitive nociceptors. The bottom traces could represent either a
non-nociceptive mechanoreceptor or a mechanosensitive capsaicin-insensitive
nociceptor. The vertical bars represent a F of 0.1. (B) Three different
cell types with a differential response to cold, menthol and capsaicin,
showing the presence of both innocuous cool and noxious cold receptors, as
well as a cold responsive cell not expressing TRP channels. The vertical bars
represent a F of 0.05. Abbreviations: bNCSC, boundary cap neural crest
stem cell; DRG, dorsal root ganglion; Hypoosm, 30% hypoosmolarity; KCl, 100 mM
potassium chloride.
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