DEV01833 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Sequence conservation of upstream elements of the Brn1 gene. The mouse (m) DNA sequences of elements A-D are shown together with their positions relative to the start codon. Only the divergent nucleotides of the human (h) Brn1 sequence are indicated. A conserved octamer sequence and the functional Pax2/5/8-binding site Dd are boxed.

• Supplemental Figure 2 -

  Fig. S2. Brn2 and Brn4 expression in the developing mid-hindbrain region. Control (Pax2^+/+ or Pax2^+/–) and Pax2^–/– embryos at the eight-somite stage were analyzed for expression of the Brn2 (Pou3f2) and Brn4 (Pou3f4) genes. Brn2 expression was downregulated in the mid-hindbrain region of Pax2^–/– embryos in comparison to the expression in rhombomere 4 (r4), which was similar in both control and Pax2^–/– embryos. Brn4 was only weakly expressed in a Pax2-independent manner in the mid-hindbrain region, whereas Tst1 (Pou3f1), the fourth member of the class III Pou gene family, was not expressed in this developing brain region (data not shown). An arrowhead indicates the position of the mid-hindbrain boundary.

• Supplemental Figure 3 -

  Fig. S3. Brn1 expression in wild-type and Pax5^–/– embryos. (A) Initiation of Brn1 expression during early somitogenesis in wild-type mouse embryos. s, somites. (B) Normal Brn1 expression in Pax5^–/– embryos at E9.5. Brn1 transcripts were detected by whole-mount in situ hybridization. fb, forebrain; mb, midbrain; hb, hindbrain. The mid-hindbrain boundary is indicated by an arrowhead.