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Fig. 7. Induction of Fgf8 in the hindbrain by ectopic expression of
EnR-Brn1. (A) Comparison of chick (c) and mouse (m) Brn1 protein sequences. A
partial chick Brn1 cDNA was PCR-cloned from embryo RNA. Numbers refer
to the corresponding amino acids of mouse Brn1, for which only the divergent
amino acids are shown. Dots indicate five gaps introduced for optimal sequence
alignment. (B,C) Brn1 expression in chick embryos at HH stages 14-15
and 19-20. (D-G) Ectopic expression of rat (r) Brn1 proteins together with
mouse Otx2 in chick embryos. The expression of endogenous chick (c)
Fgf8 (blue) and electroporated rat Brn1 (D,E,G) or chick
Pax2 (F) genes (brown) was detected by in situ hybridization.
Asterisks indicate ectopic Fgf8 expression in the hindbrain. In G,
the strong Fgf8 signal (blue) covered the Brn1 signal
(brown) because of colocalization of ectopic EnR-Brn1 and
Fgf8 expression. The upper side of the embryo is electroporated,
whereas the lower side serves as a control. (H) Hypothetical interactions that
may explain Fgf8 induction by the ectopically expressed EnR-Brn1. For
explanations, see Discussion.
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