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Fig. 8. Inhibition of RA signaling alters SVZ cell morphology and differentiation. Confocal images of 50 µm-thick resectioned explants immunolstained for GFP (green) and nestin (red in C, F) or PSA-NCAM (red in I, L). In the EGFP-electroporated control (A-C), some GFP-labeled SVZ cells express nestin (arrows) and have typical radial cell morphology. Others express GFP only (arrowheads). After GFP reporter co-electroporation with dnRAR (D-F), GFP-labeled cells lack radial morphology, have shorter processes, and more co-express nestin (arrows). Arrowheads show GFP single-labeled cells. G-L: In a typical control explant (G-I), most GFP+RMS cells express PSA-NCAM (arrows) and have a migratory morphology (fusiform shape and leading/lagging processes); in contrast, RMS cells co-expressing GFP and dnRAR (J-L) have more irregular cell bodies (arrows); many appear outside of the RMS and lack PSA-NCAM expression (arrowheads). Scale bar: 25 µm.

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