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Fig. 6. Blockade of cell-cycle progression by a CDK inhibitor, Ink4D (p19), does
not affect the inhibitory effect of Wnt2b on ganglion cell differentiation.
(A-D) Effect of Ink4D and Wnt2b on cellular proliferation. Optic vesicles of
E1.5 embryos were electroporated with vectors, as indicated above. After 12
hours, the sections were stained for BrdU (purple) and for co-electroporated
nlsGFP (green). Note that Ink4D-electroporated cells did not incorporate BrdU
in B and D. (E-L) Expression patterns of neurofilament (purple in E-H) and
islet1 (purple in I-L) in the retina 48 hours after the electroporation.
Arrowheads indicate the cells expressing co-electroporated GFP and the retinal
ganglion cell markers. Wnt2b inhibited ganglion cell differentiation in both
the presence and absence of co-electroporated Ink4D (G,H,K,L). (M) Percentage
of BrdU+ cells in retina (n=4) expressing each construct. (N) Ratio
of islet1+ cells/co-electroporated nlsGFP in the retina (n=4). Scale
bars: 20 µm (A-D); 30 µm (E-L).
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