|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 6. Induction of glandular cell-specific gene expression by CNIC.
Stage 28 PV were transfected with TP-1Venus reporter (control) or
CNIC and cultured for 36 hours. Serial sections were made from each
explant. Gene expressions were also compared with normally developing PV
(right three columns; sections in each column are also serial with Figs
1 and
7). All sections hybridized
with the same probe were placed on the same slide glass to compare intensity
of signals. Wide and efficient activation of Notch signaling is monitored by
co-transfection of Venus reporter construct in the neighboring or
next neighboring sections (inset in B and not shown). Reporter activity in
control explants is also shown (inset in A; neighbor section of A). (B)
CNIC transfection induces Smad8 expression. (G,L,Q,V)
Expression of chicken SP and of genes that are specifically
downregulated in gland cells is repressed. More than 12 explants were
investigated in three experiments. More than 15 sections made from each
explant were investigated for each probe. No obvious difference between
explants was observed. Scale bar: 100 µm.
|
