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Fig. 4. vertigo (vrt) mutants show severely delayed innervation
of the tectum. Analysis of retinal axon projection in wild type (A,C,E,G) and
vrt mutants (B,D,F,H). (A-F) Lateral views of the retinotectal
projection in Brn3c:mGFP transgenic fish, labeled with anti-GFP. At 3
dpf, the wild-type tectum (A) is fully innervated, while the vrt
tectum (B) is devoid of axons. Broken lines outline the tectum boundaries. In
4 dpf wild-type larvae (C), the density of axon arbors is increased compared
with 3 dpf (A), and dorsal and ventral branches are clearly visible in the
optic tract. In vrt (D), axons have invaded the anterior tectum, and
the optic tract (OT) is abnormally wide (arrowheads). At 6 dpf, axons
innervate the whole tectum in the vrt mutant (F) similar to wild type
(E) The optic tract remains wider than normal. (G,H) Dorsal views of 48 hpf
retinas and optic nerves, labeled with zn5. The number of RGCs and their axons
is similar between wild type (G) and vrt (H). Scale bars: 50
µm.
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