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Fig. 2. Apical capping is specific to anterior polar cells. (A) Overexpression of
the polar cell fate repressor eya. The polar cell marker Fas2 is
shown in red. (B,C) Enlarged views of the framed regions shown in A. Note the
absence of anterior polar cells and an apical cap (compare with
Fig. 1B-D). Egg chambers show
Laminin A (green) and nuclei (blue). (D-I) Mosaic egg chambers containing
eya mutant follicle cells making ectopic polar cells (Fas2, in red).
(D-F) Anterior stage 8 egg chamber with two normal polar cells and their
apical cap (D, arrowheads 1, 2). (E) A ectopic polar cell close by shows a
nascent apical cap (arrowhead 3). (F) Merged image of D and E. (G-I) A group
of five to six ectopic polar cells have assembled two separate and large
apical caps (arrowheads 1, 2). (I) Merged image of G and H. Scale bars: in A,
10 µM; in B, 10 µM for B,C; in D, 5 µM for D-F.
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