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Fig. 7. Cells within S-shaped bodies die in Fgf8 hypomorphs. (A-D)
Immunofluorescence assay for PAX2 (green), to identify the developing nephrons
and collecting ducts, and LysoTracker (LysoT, red) staining to identify
regions containing dying cells in vibratome sections of normal and
Fgf8 severe hypomorph kidneys at the stages indicated.
(A'-D') Higher magnification views. Arrows indicate regions where
Bowman's capsule progenitors are dying in normal and mutant kidneys; white
arrowhead indicates regions where tubule progenitors are dying in the mutant
kidney; open arrowheads point to regions in the peripheral zone of the mutant
kidney where cells are dying.
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