Supplemental Figure 1
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Fig. S1. Limb
outgrowth and somite development is unaffected in T-Cre; Fgf8Flox/D2,3 progeny. (A-D) Scleraxis expression in the somites
of E11.5 control (A) and mutant (C) embryos, and in the rib region of E14.5
control (B) and mutant (D) embryos. (E,F) Skeletal preparations of forelimb
(top) and hindlimb (bottom) in control (E) and mutant (F) embryos. (G) Skeletal
preparation (without heads or limbs) of control (left) and mutant (right)
neonates.
Supplemental Figure 2
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Fig. S2. Branching
morphogenesis is disrupted in T-Cre; Fgf8Flox/D2,3 kidneys. Kidneys from control (A,C,E) and T-Cre; Fgf8
Flox/D 2,3 (B,D,F) progeny probed by immunohistochemistry for
UB marker cytokeratin at E12.5 (A,B), E14.5 (C,D) and E18.5 (E,F) demonstrate
diminished UB branching in mutant tissues starting at E14.5. Scale bar: 100 mm.
Supplemental Figure 3
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Fig. S3.
Proliferation patterns are not affected in T-Cre; Fgf8Flox/D2,3 kidneys. Control (A,C,E) and mutant (B,D,F) kidneys
at the stage indicated were probed by immunohistochemistry (red) for
phosphorylated histone H3, which occurs in mitotic cells from prophase through
telophase (Brenner et al., 2003). Tissue was counterstained with DAPI (blue).
Note that the labeled pattern is approximately the same in controls and
mutants.
Supplemental Figure 4
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Fig. S4. Semi-quantitaive
RT-PCR performed in RNA isolated from E12.5 metanephric mesenchyme of control
(CM) or mutant (MM) embryos. In the left panels, the assay was performed on RNA
isolated from samples immediately after dissection, and, in the right panels, on
RNA from samples incubated with Fgf8 for 4 hours (MM+Fgf8) or in media
(MM-Fgf8). Primer sequences are available upon request.
