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Fig. 4. Wnt6 activates the ß-catenin pathway. Projections of confocal dorsal
views (A,B,E,F,I,J) and confocal transverse sections (C,D,G,H,K,L) of somites
electroporated with GFP (A-D), DN ß-catenin (E), DN Lef1 (F-H), DN Lef1
and GFP (1:1, J), DN Lef1 and paraxis (1:1, J-L). Sections are stained with a
combination of phalloidin red, recognising F-actin (in red) and an antibody
directed against N-cadherin (in blue), together with the GFP (in green).
Sections in D,H,L show the N-cadherin staining only. (A-D) Control, GFP
electroporated somites with typical long bottle-shaped cells (arrowheads in A)
that display the adherens junction-specific markers F-actin and N-cadherin at
their apical end (C,D). (B) Enlargement of cells in A. (E-H) Dermomyotome
cells electroporated with DN ß-catenin (E) or DN Lef1 (F-H) display a
round morphology with a clear redistribution of the adherens junction markers
at the plasma membrane (G,H). (J-L) Dermomyotome cells expressing DN Lef1
together with paraxis display a normal epithelial-like morphology (J) with
adherens-junction marker at the apical end (K,L). (M) Quantification of
experiments shown in A-L, where coloured bars represent the percentage of
epithelial and round cells in somites electroporated with GFP only (yellow
bar), DN Lef1 and GFP (blue bar), or a combination of DN Lef1 and paraxis
(purple bar). After GFP electroporation, only 1.5% of the cells displayed a
round morphology. The electroporation of DN Lef1+GFP increases the number of
round cells to 62.5%, while the co-electroporation of DN Lef1 and paraxis
significantly rescues the epithelial morphology, as only 24.8% of the cells
display a round morphology. Standard deviations are indicated.
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