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Fig. 1. Expression of msh and ara/caup in the wing disc. Images
show accumulation of Msh (green), Ara/Caup (red) and Wg (blue). (A) Late
second instar disc. The border between the Msh and Ara/Caup domains is
relatively sharp (arrowhead). (B) Mid-third instar discs. There is low level
Msh accumulation in the dorsal wing pouch (arrow) and in the posterior notum
(arrowhead). (C) Late third instar disc images. Channels showing Msh and
Ara/Caup expressions are separately illustrated at bottom of the panel. Yellow
lines indicate the positions of z-axis optical sections. These are
shown at left (top) and right (middle) of the panel, with separate and merged
red and green channels. White curved lines highlight the profiles of the disc
epithelium sections. vh, ventral hinge, pl, pleura; dh, dorsal hinge; n,
notum; ln, lateral notum; mn, medial notum; wp, wing pouch; a, anterior; p.
posterior; white asterisks, tegula; blue asterisks, dorsal radius.
msh expression is maximal in the dorsal and ventral prospective
hinges (white arrowheads) and lower in the posterior notum (white arrow),
while that of ara/caup is maximal at the prospective lateral notum
(yellow arrowhead). Blue arrow indicates the region of decreased Iro-C
expression in the lateral notum of late third instar discs. Blue horizontal
line indicates the fold that approximately coincides with the notum/hinge
boundary. In the notum/dorsal hinge z-section, there is strong notal
expression of ara/caup that does not overlap with the strong
expression of msh. This expression is also contiguous but does not
overlap at the z-section through the ventral hinge and pleura (top,
left). Wg marks the wing pouch epithelium (blue arrowhead) and this is
contiguous with the domain of msh expression. Therefore, this should
correspond to the ventral hinge and ara/caup expression should
correspond to the pleura.
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