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Fig. 4. Cytotrophoblasts avoid substrates containing EPHB4, but not ephrin B2.
Laminin with either ephrin B2-Fc (A) or EPHB4-Fc (B) and fluorescent
anti-human Fc was spotted on the plate before the entire surface was covered
with laminin alone. (C,D) Cytokeratin 7 staining showed cytotrophoblast
distribution on the tissue culture substrate. Initially, cytotrophoblasts were
plated as a monolayer. (C) By 12 hours, the cells continued to associate with
ephrin B2-Fc substrates, but (D) few cells were attached to EPHB4-Fc spots.
Scale bar: 200 µm.
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