|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 5. Expression pattern of mls-2 in wild-type, cc615 and
tm252 hermaphrodites. (A,B,E,F,M) Wild-type embryos (A,B) and L1
larvae (E,F,M) stained with anti-MLS-2 antibody (A,E,M) or DAPI (B,F). (C,G,N)
Expression pattern of a functional GFP::MLS-2 fusion in wild-type embryos (C)
and early L1 larvae (G,N). (D,H) Corresponding DIC images for panels C and G.
Nuclear localized MLS-2 protein was seen in a subset of cells during
embryogenesis (A,C), in six head neurons (arrowhead in E; the neuronal signal
was out of focus in G), in the M mesoblast (arrows in E and G) and in the
eight M descendants (M,N; only four cells visible in this focal plane). No
MLS-2 protein was detected after the 8-M stage (n>50). Note that
the other signals shown in G are due to gut autofluorescence. (I-L)
cc615 (I,J) and tm252 (K,L) L1 larvae stained with
anti-MLS-2 antibody (I,K) or DAPI (J,L). (I) No MLS-2 staining was detected in
cc615 mutants (n>50). (K) The MLS-2(gf) protein in
tm252 mutants was detected in the 16 M descendants (eight visible in
this focal plane) and the head neurons (data not shown). (O) GFP signal from a
mls-2p::gfp transgene in an animal with 13 M descendants (six
GFP-expressing cells visible in the focal plane shown). The GFP signal is
primarily cytoplasmic. (P-U) Transgenic animals carrying an integrated
hlh-8p::lacZ reporter were double-stained with both anti-MLS-2 (P,S)
and anti-ß-galactosidase (Q,T) antibodies. (R,U) Merged images. MLS-2
protein was detected in the M mesoblast (P-R), but not in the SMs (S-U). (V)
Summary of mls-2 expression pattern in the M lineage. The wild-type M
lineage with overlay of mls-2 expression highlighted in red. Solid
red lines indicate mls-2 expression detected by both GFP::MLS-2 and
anti-MLS-2 antibodies; dashed red lines indicate mls-2 expression
detected only by a transcriptional mls-2p::gfp reporter.
|
