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Fig. 1. Meis expression and regulation is conserved during axolotl embryogenesis. (A-D) Whole-mount in situ hybridization of late tail bud (A, lateral view) and prehatching (C, dorsal view) axolotl embryos, compared with E9.5 (B) and E10.5 (D) murine embryos, showing Meis2 expression in the head. (E-H) Meis2 in situ hybridization (E,F) and immunohistochemistry using Meis-a antibody (G,H) on adjacent stage (St) 41 sagittal hindbrain (E,G) and eye sections (F,H). (I-L,N-Q) Analysis of Meis expression during axolotl limb development. Meis-a immunohistochemistry (I-L) and in situ hybridization with a Meis2 riboprobe (N-Q) on adjacent cryosections from St36 (I,N), St40 (J,O) and St43 (K,L,P,Q) limb buds. (L,Q) Limb bud sections from a St43 axolotl larva treated with 0.12 mM retinoic acid (RA) for 22 hours. Arrowheads indicate the distal limit of nuclear Meis expression in the limb bud. (M) Anti-Pbx1/2/3/4 immunohistochemistry on a St42 limb section. Arrowhead indicates the limit between proximal high and distal low nuclear Pbx expression. (R) Negative control for immunohistochemical stainings. hb, hindbrain; mb, midbrain; R1, rhombomere 1; R2, rhombomere 2; tel, telencephalon.

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