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Fig. 4. Meis overexpression induces distal-to-proximal relocation of blastema cells. (A,E) Mid-humerus medium bud blastemas 1 day after focal electroporation of EGFP (A) or Meis2+Pbx1+EGFP (E). (B,F) The same limbs after complete regeneration. (C,G) Distal early bud blastemas one day after bulk electroporation with EGFP or EGFP+Meis1a. (D,H) The same limbs after complete regeneration. (I) Proportion of limbs showing GFP-positive cells in the different PD limb segments after focal electroporation of EGFP or Meis2±Pbx1+EGFP. (J) Percentage of GFP-positive cells in each PD segment of the limbs shown in I. (K) Percentage of GFP-positive cells within the different PD segments after focal electroporation of EGFP or Meis2±Pbx1+EGFP in the distal region of a late-bud proximal blastema.





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