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Fig. 5. Anti-Meis1 and anti-Meis2 morpholinos specifically block axolotl Meis1 and Meis2 translation. Meis1 or Meis2 constructs were co-electroporated together with anti-Meis1 or anti-Meis2 fluorescein-tagged MOs into the neural tube. Sagittal neural tube sections show MO-positive cells in green (A,D,G,J), Meis in red (B,E,H,K), and both signals merged with nuclear staining in blue (C,F,I,L). Arrowheads indicate Meis-positive cells. When Meis1 MO was co-electroporated with the Meis1 expression plasmid, no Meis1 protein expression was detected in MO-loaded cells (A-C). Instead, when Meis1 MO was co-electroporated with the Meis2 expression plasmid, MO and Meis2 protein signals were found in the same cells (D-F). Reciprocally, when Meis2 MO was co-electroporated with the Meis1 expression plasmid, MO and Meis2 protein signals were found in the same cells (G-I). Instead, when Meis2 MOs were co-electroporated with Meis2 expression plasmid, no Meis2 protein expression was detected in MO-loaded cells (J-L). These results indicate the effectiveness and specificity of the Meis1 and Meis2 MOs.

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